The Fate of Newly Synthesized Hormone from Neuroendocrine Cells of Aplysia
Identifieur interne : 000859 ( Main/Exploration ); précédent : 000858; suivant : 000860The Fate of Newly Synthesized Hormone from Neuroendocrine Cells of Aplysia
Auteurs : Wenjau Lee ; Nancy L. WayneSource :
- General and Comparative Endocrinology [ 0016-6480 ] ; 1997.
English descriptors
- Teeft :
- Abdominal ganglia, Abdominal ganglion, Academic press, Afterdischarge, Anisomycin, Anisomycin treatment, Aplysia, Aplysia californica, Californica, Cell activities, Cell afterdischarge, Cell cluster, Cell clusters, Cell content, Cell control, Cell homogenates, Cell neurons, Cell preparation, Cell preparations, Cell system, Cellular regulation, Compound action potentials, Consecutive days, Control period, Control sample, Copyright, Culture medium, Daily afterdischarges, Daily stimulation, Degradation, Electrical afterdischarge, Electrical stimulation, Endocrinology, Experimental design, Experimental period, Experimental sample, Ganglion, Glucose, Growth hormone, High rate, Hormone secretion, Hormone synthesis, Inhibitor, Intermediate products, Kaczmarek, Linear regression analysis, Lower values, Major role, Membrane excitability, Neuroendocrine, Neuroendocrine cells, Neuron, Other cluster, Other hand, Overall pattern, Pancreatic beta cells, Parathyroid hormone, Peptide, Percentage release, Physiol, Preferential release, Present study, Prohormone, Protein synthesis, Protein synthesis inhibitor, Radiolabeled, Relative contribution, Releasable, Room temperature, Same concentration, Sample collection, Scheller, Secretion, Secretory, Secretory granules, Secretory material, Successive days, Suction electrode, Sympathetic ganglion, Total amount, Total amounts, Ucla school.
Abstract
Abstract: The neuroendocrine bag cells fromAplysiacontrol reproductive functions through the regulated release of egg-laying hormone (ELH). The purposes of this study were to investigate (1) if synthesis of ELH in bag cells compensates for the release and degradation of the hormone, (2) the ratio of released to stored ELH, (3) the fate of newly synthesized ELH and its relative contribution to the overall pattern of ELH secretion, and (4) the consequences of blocking protein synthesis in bag cells on the pattern of ELH secretion and subsequent reproductive function. Excised bag cells were incubated with [3H]leucine to radiolabel newly synthesized ELH and were then electrically stimulated to trigger secretion of the hormone on 4 successive days, mimicking bag cell activities during the breeding season. Samples of bag cell secretion were collected; total (new and old) and radiolabeled (new) ELH levels were determined. The results showed that ELH synthesis could not keep up with the daily loss of ELH through release and degradation, but the ratio of released to stored ELH remained constant over the 4-day study. In addition, releasable ELH was secreted preferentially within 24 hr after it was synthesized. Blocking protein synthesis resulted in a 50% decrease in total ELH released, suggesting that newly synthesized ELH contributes to half of the total ELH secreted. Furthermore, this lowered level of total ELH, i.e., older ELH alone, was not sufficient to stimulate egg laying inAplysia,indicating that the contribution of newly synthesized ELH was physiologically relevant.
Url:
DOI: 10.1006/gcen.1997.6916
Affiliations:
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The purposes of this study were to investigate (1) if synthesis of ELH in bag cells compensates for the release and degradation of the hormone, (2) the ratio of released to stored ELH, (3) the fate of newly synthesized ELH and its relative contribution to the overall pattern of ELH secretion, and (4) the consequences of blocking protein synthesis in bag cells on the pattern of ELH secretion and subsequent reproductive function. Excised bag cells were incubated with [3H]leucine to radiolabel newly synthesized ELH and were then electrically stimulated to trigger secretion of the hormone on 4 successive days, mimicking bag cell activities during the breeding season. Samples of bag cell secretion were collected; total (new and old) and radiolabeled (new) ELH levels were determined. The results showed that ELH synthesis could not keep up with the daily loss of ELH through release and degradation, but the ratio of released to stored ELH remained constant over the 4-day study. In addition, releasable ELH was secreted preferentially within 24 hr after it was synthesized. Blocking protein synthesis resulted in a 50% decrease in total ELH released, suggesting that newly synthesized ELH contributes to half of the total ELH secreted. Furthermore, this lowered level of total ELH, i.e., older ELH alone, was not sufficient to stimulate egg laying inAplysia,indicating that the contribution of newly synthesized ELH was physiologically relevant.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Lee, Wenjau" sort="Lee, Wenjau" uniqKey="Lee W" first="Wenjau" last="Lee">Wenjau Lee</name><name sortKey="Wayne, Nancy L" sort="Wayne, Nancy L" uniqKey="Wayne N" first="Nancy L." last="Wayne">Nancy L. Wayne</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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